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Immune-fluorescence images of a submandibular lymph node containing a 7mm deposit of poorly differentiated SCC. This includes (A) whole slide scans (WSS) and (B) Representative regions of interest (ROI) showing merge and single staining for panel 1: CD3 (red), CD4 (yellow), CD8 (green), granzyme B (magenta), CD103 (cyan), and panCK (orange) antibodies with DAPI (blue) counterstain (upper immune-fluorescence panel); and panel 2: CD3 (red), PD-1 (green), PD-L1 (magenta), <t>CD68</t> (cyan), MHC class II (yellow) and panCK (orange) antibodies with DAPI (blue) counterstain (lower immune-fluorescence panel). All images were taken at 20x magnification, and scale bars indicate 100μm.
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Immune-fluorescence images of a submandibular lymph node containing a 7mm deposit of poorly differentiated SCC. This includes (A) whole slide scans (WSS) and (B) Representative regions of interest (ROI) showing merge and single staining for panel 1: CD3 (red), CD4 (yellow), CD8 (green), granzyme B (magenta), CD103 (cyan), and panCK (orange) antibodies with DAPI (blue) counterstain (upper immune-fluorescence panel); and panel 2: CD3 (red), PD-1 (green), PD-L1 (magenta), CD68 (cyan), MHC class II (yellow) and panCK (orange) antibodies with DAPI (blue) counterstain (lower immune-fluorescence panel). All images were taken at 20x magnification, and scale bars indicate 100μm.

Journal: Frontiers in Immunology

Article Title: Case report: Partial regression of metastatic squamous cell carcinoma with altered azathioprine dosage after long-term use in renal transplant patient

doi: 10.3389/fimmu.2024.1474663

Figure Lengend Snippet: Immune-fluorescence images of a submandibular lymph node containing a 7mm deposit of poorly differentiated SCC. This includes (A) whole slide scans (WSS) and (B) Representative regions of interest (ROI) showing merge and single staining for panel 1: CD3 (red), CD4 (yellow), CD8 (green), granzyme B (magenta), CD103 (cyan), and panCK (orange) antibodies with DAPI (blue) counterstain (upper immune-fluorescence panel); and panel 2: CD3 (red), PD-1 (green), PD-L1 (magenta), CD68 (cyan), MHC class II (yellow) and panCK (orange) antibodies with DAPI (blue) counterstain (lower immune-fluorescence panel). All images were taken at 20x magnification, and scale bars indicate 100μm.

Article Snippet: Primary antibody specifications included CD3 (SP7, ab16669, Abcam), CD4 (EPR6855, ab133616, Abcam), CD8 (C8/144B, MA5-13473, Invitrogen), CD103 (EPR4166 , ab129202, Abcam), granzyme B (D6E9W, 46890, Cell signaling), CD68 (PGM1, MA5-12407, Invitrogen), PD-1 (NAT105, ab52587, Abcam), PDL1 (E1L3N, 13684, Cell signaling), MHC class II (HLA-DR/DP/DQ/DX) (CR3/43, sc-53302, Santa Cruz) and panCK (AE1/AE3, Akoya Biosciences ® ).

Techniques: Fluorescence, Staining